Reporter

Part:BBa_K2525023:Design

Designed by: Kshitij Rai   Group: iGEM17_IIT_Delhi   (2017-10-27)


PsrpR + RBS + GFP + T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 738


Design Notes

Since the part was RFC10 compatible, no significant design issues were faced.


Source

The SrpR repressible promoter comes from the genomic sequence of Pseudomonas putida

References